Molecular typing of clinical isolates of Mycobacterium tuberculosis refereed to the west tuberculosis center by PFGE method

BACKGROUND AND OBJECTIVESTuberculosis (TB) is a highly contagious and chronic infectious disease. Nowadays, TB is causative a public health issue in the global. Molecular typing information is important role for ascertain the genotypic diversity of M. tuberculosis strains and to control infectious diseases transmission and in the prevention of the tuberculosis spread differentiation between new infections, and relapses that can effectively control the infections. The study aimed to compare the ability of restriction enzymes to detection the genetic relevance between strains and antimicrobial resistance profiles among Mycobacterium tuberculosis isolated from patients.MATERIALS AND METHODSTotally, ۷۴ M. tuberculosis isolates were collected from patients referred to the tuberculosis center. Identification of all isolates was accomplished by standard biochemical methods. drug susceptibility test was performed using the commensurate method then the bacteria cells be embedded in a plug of agarose،PFGE plaques were digested by, XbaI, DraI restriction enzyme. Finally, the digested DNA fragments were separated on ۱% agarose gel and analyzed by Bio Numerics software.RESULTS AND DISCUSSIONThirteen (۱۷.۶%) out of ۷۴ isolates were MDR, mostly related to Kermanshah patients; ۵۹ isolates were digested with DraI enzyme and ۵۳ isolates with XbaI enzyme. The discriminatory power of DraI (۸ pulsotypes) is slightly higher than XbaI (۴ pulsotypes).CONCLUSIONGenotyping methods to demonstrate the spread of resistant M. tuberculosis isolates is important. The PFGE is considered as a powerful and useful tool to study the genetic diversity of many microscopic species. Therefore, this method is suggested as complementary techniques for molecular epidemiological studies.

کلیدواژه ها:

Molecular typing ، Mycobacterium tuberculosis ، Polymerase chain reaction ، Pulsed‑field gel electrophoresis