Majid Rahmati ۱ - ۱ Dept. of medical Biotechnology, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Rahele Khademi ۲ - ۲ Dept. of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Zohreh Sharifi ۳ - ۳ Iranian Blood Transfusion Organization Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
Fatemeh Yari ۳
Mohsen Badalzadeh ۴ - ۴ Immunology, asthma and allergy research institute, Tehran University of Medical Sciences, Tehran, Iran.
Maryam Taheri ۵ - ۵ Information technology office, Iranian blood transfusion organization (IBTO), Tehran, Iran.
Reyhaneh Khademi ۳*

Background: Platelet MPs are produced automatically during platelet storage. Their size is about ۰.۱ – ۱ µm and have ability for sub- endothelium adhering, strengthen of platelet aggregation and also platelet pre- coagulation. In fact, the release of membrane particles helps to signal to the neighbor cells or to seclude target cells from apoptosis. The purpose of this study is to determine the effect of time passing on platelet MPs production.Methods: In this experimental study, ۱۲ units of PC from healthy donors were obtained. Sampling was done at ۲, ۳, ۵ and ۷ days after storage. After separating of MPs, they were prepared for flow cytometery analyses by anti- CD۴۱a FITC and assessed their concentration through Bradford methods. Paired sample T-test and ANOVA were used to compare the results and investigate the probable significant differences of this experiment.Results: There was no significant difference in platelet MPs concentration between PC stored in day ۲ and ۳. (P> ۰.۰۵) On the other hand, we could claim significant differences in platelet MPs concentration between PC stored in day ۳ and day ۵ and also between day ۵ and day ۷. (P< ۰.۰۵)Conclusions: We showed that the production or releasing of platelet MPs were considerable by passing time. Also by increasing of storage time, platelet component included more MPs which were able to signal and produce interaction with other cells in patient receiving bodies. As a result, it was possible to interfere in their immune responses, inflammatory or coagulation processes after transfusionBackground: Platelet MPs are produced automatically during platelet storage. Their size is about ۰.۱ – ۱ µm and have ability for sub- endothelium adhering, strengthen of platelet aggregation and also platelet pre- coagulation. In fact, the release of membrane particles helps to signal to the neighbor cells or to seclude target cells from apoptosis. The purpose of this study is to determine the effect of time passing on platelet MPs production. Methods: In this experimental study, ۱۲ units of PC from healthy donors were obtained. Sampling was done at ۲, ۳, ۵ and ۷ days after storage. After separating of MPs, they were prepared for flow cytometery analyses by anti- CD۴۱a FITC and assessed their concentration through Bradford methods. Paired sample T-test and ANOVA were used to compare the results and investigate the probable significant differences of this experiment. Results: There was no significant difference in platelet MPs concentration between PC stored in day ۲ and ۳. (P> ۰.۰۵) On the other hand, we could claim significant differences in platelet MPs concentration between PC stored in day ۳ and day ۵ and also between day ۵ and day ۷. (P< ۰.۰۵) Conclusions: We showed that the production or releasing of platelet MPs were considerable by passing time. Also by increasing of storage time, platelet component included more MPs which were able to signal and produce interaction with other cells in patient receiving bodies. As a result, it was possible to interfere in their immune responses, inflammatory or coagulation processes after transfusion

Platelet, Microparticle, Hemostasis, Thrombosis, Inflammatory.